Small polydisperse circular dna of hela cells

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August undefined, 2024

WebSep 3, 2024 · Smith CA, Vinograd J. Small polydisperse circular DNA of HeLa cells. J Mol Biol. 1972;69:163–78. Article CAS PubMed Google Scholar Stanfield S, Helinski DR. Small circular DNA in Drosophila melanogaster. Cell. 1976;9:333–45. Article CAS PubMed Google Scholar Wong FY, Wildman SG. WebCircular DNA 作者: 期刊: Nature ... Electron microscopy on preparations from HeLa cells reveals, in addition to the circular molecules previously encountered in mitochondria (mean length 4.8 microns), some large supercoils which are multiples of these, and also some very small and polydisperse circles (0.2-3.5 microns). These could code ...

cccDNA - Wikipedia

Webof populations of DNA molecules of essentially identical sequence. The most thoroughly characterized spcDNA is that from three established cell lines: Drosophila Schneider's line 2 cells (8,9), AGM BSC-1 cells (11,12,13), and HeLa cells (10). The small circular DNAs from Schneider's cells have a mean length of 3.3kh and range in size from ... WebSep 3, 2024 · EccDNAs with a length less than 500 bp are called small polydisperse circular DNA (spcDNA) [17, 29–32], and a few spcDNA molecules are hybridized to unique sequences [29, 31–33]. The eccDNA distribution is not restricted to … crispin mariano

Extrachromosomal circular DNA in cancer: history, current …

WebApr 12, 2024 · Of note, the cells were exposed to different stresses after 24 h of initial cell seeding, challenging HeLa cells as follows: Staurosporine and Rapamycin were administrated at a final concentration of 10 µM and 20 nM, respectively, whereas H 2 O 2 was administrated to reach 50 µM in the cell solution. For the hypotonic stress, the cells … WebClosed covalent circular DNA (cccDNA) is a unique DNA structure that forms in response to infection of a cell. Genomic DNA enters the cell nucleus, and partially double-stranded … WebSmall polydisperse circular DNA of HeLa cells (Q70476021) From Wikidata. Jump to navigation Jump to search. scientific article published on 01 August 1972. edit. Language … crispin lipscomb

Telomeric repeats on small polydisperse circular DNA …

Small extrachromosomal circular DNA (eccDNA): major functions …

WebHuman Genetics 91 , 489–495 ( 1993) Cite this article 56 Accesses 16 Citations Metrics By screening total human DNA with probes derived from the small polydisperse circular (spc) DNA fraction of cultured human cells, we identified three clones that carry long stretches of β-satellite DNA. crispin imagingWebNov 1, 1984 · RESULTS (a) Purification of cccDNAs HeLa cells contain a small amount of spcDNA, amounting at maximum to 0.02% of the total cellular DNA, even after the … mandala semplici da colorare e stampare

"WebThe size of small polydisperse circular DNA (spcDNA) in angiofibroma-derived cell cultures from patients with tuberous sclerosis (TSC) differs from that in fibroblasts. Human … " - Small polydisperse circular dna of hela cells

Small polydisperse circular dna of hela cells

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WebJul 1, 1994 · Characterization of cloned human alphoid satellite with an unusual monomeric construction: evidence for enrichment in HeLa small polydisperse circular DNA. Nucleic Acids Res. 1985 Feb 11; 13 (3) ... Smith CA, Vinograd J. Small polydisperse circular DNA of HeLa cells. J Mol Biol. 1972 Aug 21; 69 (2):163–178. [Google Scholar] WebIntroduction. Extrachromosomal circular DNA (eccDNA) is a type of cell free DNA (cfDNA) which is more structurally stable than linear cfDNA currently used for cancer-related detections under clinical settings. 1,2 Although eccDNA was first discovered in 1965 by Hotta and Bassel et al in boar sperm using an electron microscope, further research on …

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WebApr 1, 2024 · technique to isolate closed circular DNA. The genomic DNA is mixed in a CsCl solution containing ethidium bromide (an intercalating agent) and subjected to ultracentrifugation to form a density gradient. Plasmid–DNA sample is run separately to locate where circular DNA will sediment. WebDec 31, 1998 · Abstract. Small polydisperse circular DNA (spcDNA) is a heterogeneous population of extrachromosomal circular molecules present in a large variety of …

WebExtrachromosomal circular DNA (eccDNA) is a closed-circle, nuclear, nonplasmid DNA molecule found in all tested eukaryotes. eccDNA plays important roles in cancer … WebSmall polydisperse circular DNA of HeLa cells. Smith CA; Vinograd J J Mol Biol; 1972 Aug; 69(2):163-78. PubMed ID: 5070865 ... Effect of ethidium bromide on mitochondrial DNA and cytochrome synthesis in HeLa cells. Radsak K; Kato K; Sato N; Koprowski H Exp Cell Res; 1971 Jun; 66(2):410-6. PubMed ID: 5556428

WebOct 14, 2024 · Ectopic expression of Gal-7 in HeLa cells renders the cells more sensitive to a variety of apoptotic stimuli . Juan Carlos Higareda-Almaraz and colleagues examined the re-expression of Gal-7 in HeLa cells, analyzed their transcriptome, and found multiple genes and transcriptional regulators involved in lipid metabolic regulation were modulated ... WebSep 9, 2024 · Small polydisperse circular DNA (spcDNA) was an obsolete concept to commonly characterize small eccDNAs with repetitive sequences. The length of …

WebOct 18, 2024 · A decade later Jerome Vinograd (1972)identified the small polydisperse circular DNAs in HeLa cells and showed that they originate from the chromosomal DNA. Vinograd also proposed...

http://archive.nstl.gov.cn/Archives/browse.do?action=viewDetail&articleID=f0891a987daedbab&navig=9565bcbb40dbfbe9&navigator=category&flag=byWord&subjectCode=null&searchfrom=null crispin lettsWebJan 1, 2001 · Previous studies have suggested that eccDNA is generated through a process involving recombination between repetitive sequences. However, we observed that … crispin mardonWebSmall polydispersed circular DNA (spcDNA) belongs to the extrachromosomal pool of DNA and is composed of heterogeneous DNA circles. ... Small polydispersed circular DNA … crispinillisWebOct 25, 1989 · An abundant class of HeLa extrachromosomal circular DNA containing the transposon-like element, THE-1, is shown to arise via site specific recombination. The chromosomal locus from which these circles are derived, however, is single-copy. Northern blot analysis detects homology to two polyadenylated RNAs in HeLa cells. crispin lakeWebSep 14, 2024 · Models of how extrachromosomal circular DNA is formed. a In response to environmental changes, cells could repair DNA strand break through removing the damaged segments. After then, the small DNA segments could form circular DNA. b Chromothripsis is a single-step catastrophic event that drives chromosome breakage and end-to-end … crispin last nameWebMar 8, 2012 · The circular DNA from mouse tissues and cell lines were 80 to 2000 bp long, ... et al.., Purification of small polydisperse circular DNA of eukaryotic cells by use of ATP-dependent deoxyribonuclease. ... Murnane J. P., Formation of extrachromosomal circular DNA in HeLa cells by nonhomologous recombination. Nucleic Acids Res. 22, 2447 (1994 ... mandala servicenowWebAbstract. Part I of the thesis is concerned with mitochondrial DNA (mtDNA) of animal cells. Complex mtDNA includes catenated oligomers two or more of the basic 5-μ circles linked crispin madavo